Methods Presented at Forum Event

At the Forum Event 12-14 May 2009 was a joint event of the EUprojects Forinvitox and Invitopharma and IVTIP and organised by Silverdal Science Park at Karolinska Institutet, Stockholm, Sweden. The Following models with commercial potential were shown at the Forum.

 

Organisation

Method

Contact

Presentation

Nikon Instruments Europe BV, Netherlands

Incubator with in-built robot, microscope,

imaging system and analysis software

Sundeep Bhandari

bhandari@nikonbv.nl

Nikon BioStation CT - an ”all-in-one” toxicity testing system solution

Image Nikon Instruments.pdf (546,3 kB)

Biopredic International, Rennes, France

Use of HepaRG® cells for cytotoxicity and genotoxicity tests

Christophe Chesné

christophe.chesne@biopredic.com

HepaRG® human hepatocytes for toxicology studies

Biopredic International, Rennes, France

Use of HepaRG cells for enzyme induction,

hepatic drug transport function, hepatotoxic effects

Christophe Chesné

christophe.chesne@biopredic.com

HepaRG® cell line: a new tool for drug metabolism and

chemical -induced hepatotoxicity investigation

Epithelix Sàrl, Geneva, Switzerland

Studying of human respiratory diseases and testing for effects

on human respiratory tracts

Samuel Constant

samual.constant@epithelix.com

The use of an in vitro cell model of the Human Airway Epithelium

(MucilAir™) in preclinical development

Image Epithelix.pdf (1109,7 kB)

EggCentris, Brussels, Belgium

Battery of follicle bioassay (FBA), in vitro fertilization pocedure (IVF)

and the mouse embryo assay (MEA)

Rita Cortvrindt

rita.cortvrindt@eggcentris.com

Battery of in vitro bioassays for the assessment of female fertility

EggCentris, Brussels, Belgium

Sperm Motility Assay (SMA) for detection of sperm toxicity

Murine Leydig Tumor cells (MLTC-1) and primary rat/mouse Sertoli cells

for testis specific toxicity

Rita Cortvrindt

rita.cortvrindt@eggcentris.com

Battery of in vitro bioassays for the assessment of male fertility

EggCentris, Brussels, Belgium

Mouse Embryo Peri-Implantation Assay (MEPA) using mouse zygotes cultured

to day 8 of development to study effects on early embryo development.

The Embryonic Stem Cell Test (EST) study post-implantation embryo toxicity

Rita Cortvrindt

rita.cortvrindt@eggcentris.com

In vitro bioassays for the assessment of pre-and post-implantation

embryo toxicity

Institute for In Vitro Sciences,

Gaithersburg, USA

Reconstructed Skin Micronucleus (RSMN) assay

Rodger D. Curren

rcurren@iivs.org

Development of a New In Vitro Genetic Toxicology Model Using

Reconstructed Human Skin

Image Institute for In Vitro Sciences.pdf (809,2 kB)

Karolinska Institutet, Sweden

Use of Small Model Organisms (SMOs) as biosensors and Xenopus and Zebrafish embryos as models to study effects of endocrine disruptors and/or medical drugs.

Manuela Dahinden-Hase

manuela.hase@ki.se

SME RECEPTOR - an industry-academia partnership for in vitro toxicology testing

Department of Neurochemistry, Stockholm

University, Stockholm, Sweden

TRPV1-expressing SH-SY5Y cells

Anna Forsby

annaf@neurochem.su.se

The in vitro nociception assay for estimation of mild eye irritation

BioMed-zet-Life Science, Linz, Austria

Expression of CYPs in HepaRG cells, cultured using a porous plasma activated scaffold

Jürgen Frank

j.frank@zet.or.at

Development of an in vitro 3D-liver test system for the investigation of drug metabolim and long term drug hepatotoxicity

Institut für Umweltmedizinische Forschung,

Duesseldorf, Germany

Neurospheres from human and mouse fetal brains as a screening system for developmental neurotoxicity

Ellen Fritsche

ellen.fritsche@uni-duesseldorf.de

Neurospheres for developmental neurotoxicity (DNT) testing

British American Tobacco, Southampton, UK

Exposure of human lung primary epithelial cells to whole cigarette smoke

Marianna D Gaca

marianna_gaca@bat.com

A model of in vitro exposure to aerosols at the air-liquid interface

SymCel, Stockholm, Sweden

Energy turnover of isolated cells, tissues or organism assessed by monitoring their heat production by calorimetry

Dan Hallén

dan.hallen@symcel.se

SymCel label-free and real-time data technology

Cellial Technologies, Lens Cedex, France

Differentiated endothelial cell monolayer

Dorothée Hallier-Vanuxeem

dvanuxeem@hotmail.com

Ready-to-use in vitro Blood-Brain Barrier model

F.C.V. Centro Investigacion Principe Felipe,

Valencia, Spain

Flow cytometry and High-Content Analysis on 96-well miniaturized assays with 3 cell lines

Guadalupe Herrera

compras@cipf.es

Multiparametric cytomic panel for in vitro cytotoxicity screening (MultiCytox-H®)

CARDAM, (VITO NV), Mol, Begium

Gene-expression (microarray, RT-PCR) in dendritic cells

Jef Hooyberghs

Jef.Hooyberghs@vito.be

VITOSENS™: a cell-based alternative to identify skin sensitizers

by gene expression

TNO Quality of Life, Zeist, The Netherlands

A human skin model applicable for genotoxicity tests

Cyrille Krul

Cyrille.Krul@tno.nl

The use of ex vivo human skin tissue cultures for safety testing

Joint Research Centre, Institute for Health

and Consumer Protection, Italy

Cultured networks of neurons (in vitro/ex vivo) for neurotoxicity evaluation

Antonio Novellino

Novellino@jrc.itj

MEA based high-troughout electrophysiology for in vitro toxicology

testing

F.C.V. Centro Investigacion Principe Felipe, Valencia, Spain

Combination of environment-independent, hermetic container to maintain

longterm cell cultures and toxicity measurements by light or fluorescence microscopy

José-Enrique O’Connor

compras@cipf.es

The use of PetakaG2™ hermetic cell culture containers and cytomics for testing of sustained in vitro toxicity (MultiCytox-LT®)

University of Vermont, Burlington VT, USA

Microscopic imaging by use of fluorescent reporter gene enabling specific organ/tissue development and metabolioc pathways

Matthew D. Rand

Matthew.Rand@uvm.edu

A Drosophila embryo platform for high-troughput testing of suspeced toxins and bioactive compounds

Solidus, Biosciences, Troy, USA

DataChip analysis for evaluation of systemic and acute toxicity

David Rozzell

rozzell@solidusbio.com

Miniaturized three-dimensional cell culture and metabolic enzyme arrays for high-throughput toxicity assays

PRIMACYT Cell culture Technology,

Schwerin, Germany

Long-term culture systems for hepatocytes for repetative analyses of metabolic and toxic effects of drugs

Dieter Runge

Dieter.Runge@primacyt.de

HEPAC: Serum-free, standardized and validated (re-usable) primary human hepatocytes for the analysis of xenobiotics

CARDAM, (VITO NV), Mol, Begium

Tail coiling and swimming behavior as identification of developmental

neurotoxicity

Ingrid Selderslaghs

Ingrid.Selderslaghs@vito.be

The zebrafish embryo as an alternative model to screen for the developmental neurotoxic potential of chemicals and drugs

CIBERESP, Barcelona, Spain and Stockholm

University, Stockholm, Sweden

GABAA receptor activity and Cell Membrane Potential in primary neuronal

cultures and the human neuronal cell line SH-SY5Y

Cristina Suñol

csenqi@iibb.csic.es

Combination of cell based assays determining GABAA receptor activity

and Cell Membrane Potential for in vitro testing of neurotoxicity

Vrije Universiteit Brussel, Belgium

Liver-based in vitro models for screening for liver toxicity

Tamara Vanhaecke Tamara.

Vanhaecke@vub.ac.be

Epigenetic modifiers as key tools to stabilise primary hepatocytes

cultures and to produce progenitor-derived hepatocytes

BioThema, Stockholm, Sweden

Measurements of intracellular ATP levels used for cyotoxicity testing

Ida Westerdahl

Ida.Westerdahl@biothema.com

ATP measurements in cell proliferation and cytotoxicity studies

Cellasys, Munich, Germany

Monitors parameters directly at living cells

Joachim Wiest

wiest@cellasys.com

Label-free cell based assays as alternative testing method for toxicity

ADVANCELL, Valencia, Spain

Reagent ISOCYP-TOX for in vitro screening of CYP450

biotranformation-mediated toxicity

Maya R. Vilá

toni@advancell.net

ISOCYP-TOX: A new ready-to-use concept for in vitro evaluation of

biotransformation-mediated toxicity